Want to keep track of the largest startup funding deals in 2025 with our curated list of $100 million-plus venture deals to U.S.-based companies? Check out The Crunchbase Megadeals Board.

This is a weekly feature that runs down the week’s top 10 announced funding rounds in the U.S. Check out last week’s biggest funding rounds here.

Investor enthusiasm for AI once again drove the bulk of funding for the week’s largest rounds, led by Anthropic’s $13 billion mega-financing. However, companies across a number of industries attracted large investments, including biotech, security and medical devices.

1. Anthropic, $13B, generative AI: San Francisco-based generative AI unicorn Anthropic raised a $13 billion Series F round at a $183 billion valuation, making it the fourth-most valuable private company in the world, per Crunchbase data. Iconiq Capital led the round, with Fidelity Management & Research Co. and Lightspeed Venture Partners co-leading.

2. Sierra, $350M, enterprise AI: Sierra, a provider of AI-enabled customer experience technology, raised $350 million at a $10 billion valuation. The San Francisco-headquartered company was founded in 2023 by former Salesforce 1co-CEO and current OpenAI board chair Bret Taylor and former Google executive Clay Bavor.

3. Treeline Biosciences, $200M, biotech: Watertown, Massachusetts-based Treeline Biosciences, a developer of medicines for cancer and other serious diseases, announced the close of a $200 million Series A extension round, bringing total funding to $1.1 billion, per Crunchbase data. The 4-year-old company also says it will begin trials for multiple cancer drugs.

4. (tied) Enveda Biosciences, $150M, drug discovery: Enveda, a startup that is working to discover new drugs by studying the chemistry of plants, raised $150 million in a Series D led by Premji Invest. The financing set a unicorn valuation for the 6-year-old, Boulder, Colorado-based company.

4. (tied) Baseten, $150M, AI: Baseten, a provider of technology for AI application developers, raised $150 million in a Series D led by Bond. The round brings funding to date for the San Francisco-based startup to $285 million, per Crunchbase data.

6. (tied) Galvenize Therapeutics, $100M, medical devices: Galvenize Therapeutics, a developer of pulsed electric field therapies for oncology and chronic lung disease, secured a $100 million Series C round. Sofinnova Partners led the financing for the Redwood City, California-based company.

6. (tied) You.com, $100M, AI: You.com locked up $100 million in Series C funding at a $1.5 billion valuation, led by Cox Enterprises. The Palo Alto, California-based company develops AI infrastructure with an eye to helping agentic AI applications access the information they need.

8. (tied) Exa, $85M, AI search: Exa, a startup that bills itself as the search engine for AI, picked up $70 million in Series B funding at a $700 million valuation. Benchmark led the financing for the 4-year-old San Francisco-based company.

8. (tied) Augment, $85M, AI logistics: San Francisco-based Augment, developer of an AI tool called Augie that was built to handle shipments for the logistics industry, landed $85 million in a Series A round led by Redpoint.

10. (tied) Shift5, $75M, cybersecurity: Arlington, Virginia-based Shift5, developer of an intelligence platform for U.S. defense and transportation systems, closed on $75 million in a Series C financing led by Hedosophia.

10. (tied) Mojo Vision, $75M, micro-LED technology: Mojo Vision, developer of a flexible micro-LED platform for use in multiple industries, raised $75 million in what it called a Series B Prime funding round. Vanedge Capital led the financing for the Cupertino, California-based company.

Methodology

We tracked the largest announced rounds in the Crunchbase database that were raised by U.S.-based companies for the period of Aug. 30-Sept. 5. Although most announced rounds are represented in the database, there could be a small time lag as some rounds are reported late in the week.

Illustration: Dom Guzman

A consortium led by Honeywell has received UK Government funding for a project that aims to revolutionise how critical aerospace technologies are manufactured in the UK through the use of artificial intelligence (AI) and additive manufacturing (AM).

The £14.1m research and development project, funded through the ATI Programme, will explore how AI and other technologies can drive simulations and modelling to lead to accelerated innovation cycles. It will also explore opportunities to increase efficiency, reduce Scope 3 emissions and help address supply chain challenges through the production of additively manufactured, qualified aerospace parts.

Project Strata will focus on the development of five components within environmental control systems (ECS) and cabin pressure control systems (CPCS) aboard aircraft.

Strata also aims to advance thermal management technology for next-generation aircraft, to reduce the amount of energy required for the heating and cooling of aircraft, as well as fuel consumption.

“Additive manufacturing has the ability to deliver both high-performance optimised components and the consolidation of complex assemblies into a single part, which can lower manufacturing costs, reduce waste and deliver new efficiencies in manufacturing,” said Jacqueline Castle, CTO at the Aerospace Technology Institute (ATI). “Strata will bring this capability to critical aircraft systems, utilising advanced simulation technologies to transform the design process. This project aligns well with the ATI’s strategy and roadmap for additive manufacturing for advanced systems, which sees additive manufacturing contributing significantly to the sector’s future sustainability.”

The consortium partners include 3T Additive Manufacturing; BeyondMath; Qdot Technology; and Oxford Thermofluids Institute, part of the University of Oxford.

The ATI Programme funds research into advanced civil aerospace technologies, and is delivered in partnership between the ATI, the Department for Business & Trade, and Innovate UK.